Generator

Part:BBa_K1486032:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-27)

sfGFP + ADH1 terminator + Ura3 cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1547
    Illegal EcoRI site found at 2478
    Illegal PstI site found at 1236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1547
    Illegal EcoRI site found at 2478
    Illegal PstI site found at 1236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1547
    Illegal EcoRI site found at 2478
    Illegal BglII site found at 1026
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1547
    Illegal EcoRI site found at 2478
    Illegal PstI site found at 1236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1547
    Illegal EcoRI site found at 2478
    Illegal PstI site found at 1236
  • 1000
    COMPATIBLE WITH RFC[1000]



Source

It was extracted by PCR from the plasmid we ordered to addgene: Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.

References